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AcaClone pDRAW32 - Freeware DNA cloning, analysis and plasmid/DNA plotting software.
http://www.acaclone.com/

ANTHEPROT - a package for protein sequence analysis.
http://antheprot-pbil.ibcp.fr/

Artemis is a DNA sequence viewer and annotation tool that allows visualization of sequence features and the results of analyses within the context of the sequence, and its six-frame translation.
http://www.sanger.ac.uk/Software/Artemis/index.shtml

BioEdit is a user-friendly sequence alignment editor which runs on Windows 95, 98 and NT.
http://www.mbio.ncsu.edu/RNaseP/info/programs/BIOEDIT/bioedit.html

CHROMA: consensus-based colouring of multiple alignments for publication.
http://www.lg.ndirect.co.uk/chroma/

Chromas is a Windows application which displays and prints chromatogram files from ABI automated DNA sequencers, and Staden SCF files which the analysis programs for ALF, Li-Cor and Visible Genetics OpenGene sequencers can create.
http://www.technelysium.com.au/chromas.html

CLC Free Workbench includes basic bioinformatics analyses and smooth data management, combined with nice graphical viewing and output options. Some analyses are alignments, ORF determination, DNA translation, phylogenies and restriction site analysis.
http://www.clcbio.com/index.php?area=software§ion=free

CLC Gene Workbench is a software suite with a range of advanced and integrated DNA sequence analysis tools - among others, assembly of DNA sequence data, advanced primer design, and molecular cloning. Available for Windows, Mac OS X, and Linux.
http://www.clcbio.com/index.php?id=27

CLC Protein Workbench is a software suite with a range of advanced and integrated protein sequence analysis tools - among others, secondary structure and signal peptide prediction, and hydrophobicity analyses. Available for Windows, Mac OS X, and Linux.
http://www.clcbio.com/index.php?id=93

Correspondence Analysis of Codon Usage
http://www.molbiol.ox.ac.uk/cu/

DNA for Windows - A compact DNA sequence analysis program, with features including sequence editing, translation, restiction site location, contig assembly, codon.
http://www.durham.ac.uk/d.p.dixon/dna.htm

DNASIS - genetic systems: plasmid map drawing, primer design, multiple sequence alignment etc. .
http://www.oligo.net/dnasis.htm

ForCon is a user-friendly software tool for the conversion of nucleic acid and amino acid sequence alignments.
http://www.hgmp.mrc.ac.uk/embnet.news/vol6_1/ForCon/body_forcon.html

Genalysis - Genome Analysis Toolkit.
http://www.genpakdna.com/genalysis/index.shtml

Lasergene 99 is the complete sequence analysis system for Windows and Macintosh
http://www.dnastar.com/

MatInspector - a tool for detection of transcription factor binding sites.
http://www.gsf.de/biodv/matinspector.html

OMIGA - sequence analysis tool featuring PCR primer prediction, multiple sequence alignments, and protein analysis. It lets you layer analysis results for comparison; organize projects, sequences, results, and datafiles.
http://www.oxmol.com/prods/omiga/

Phrap and Phred for Windows, MacOS, Linux, and Unix - Fast sequence assembly and better base calling.
http://www.phrap.com/index.htm

SeaView is a graphical multiple sequence alignment editor developped by Manolo Gouy. SeaView is able to read various alignment formats (MSF, CLUSTAL, FASTA, PHYLIP, MASE).
http://pbil.univ-lyon1.fr/software/seaview.html

SeqPup - a biosequence editor and analysis program.It includes links to network services and external analysis programs.
http://iubio.bio.indiana.edu/soft/molbio/seqpup/java/seqpup-doc.html

SEQtools - Program package for handling and analysis of nucleotide and protein sequences. In addition to many trivial functions, it includes advanced facilities for automated blast searching, sequence retrieval and design and layout of microarrays.
http://www.seqtools.dk/

SeqVerter is a free sequence file format conversion utility by GeneStudio, Inc. SeqVerter encapsulates a small subset of the features offered by GeneStudio, the complete sequence analysis software suite.
http://www.genestudio.com

Tandem repeats finder: a program to analyze DNA sequences.
http://c3.biomath.mssm.edu/trf.html

TeXshade is an alignment shading LaTeX macro package for processing multiple sequence alignments. It provides special shading modes featuring functional aspects, e.g. charge, and a plenitude of commands for handling shading colors, text styles and labels.
http://homepages.uni-tuebingen.de/beitz/tse.html

WinPep is an application for Win95/Win98/Win NT for analysis of polypeptide sequences.
http://www.ipw.agrl.ethz.ch/~lhennig/winpep.html