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CLC Free Workbench includes basic bioinformatics analyses and smooth data management, combined with nice graphical viewing and output options. Some analyses are alignments, ORF determination, DNA translation, phylogenies and restriction site analysis.
http://www.clcbio.com/index.php?area=software§ion=free

CLC Gene Workbench is a software suite with a range of advanced and integrated DNA sequence analysis tools - among others, assembly of DNA sequence data, advanced primer design, and molecular cloning. Available for Windows, Mac OS X, and Linux.
http://www.clcbio.com/index.php?id=27

CLC Protein Workbench is a software suite with a range of advanced and integrated protein sequence analysis tools - among others, secondary structure and signal peptide prediction, and hydrophobicity analyses. Available for Windows, Mac OS X, and Linux.
http://www.clcbio.com/index.php?id=93

ClustalX
http://www-igbmc.u-strasbg.fr/BioInfo/ClustalX/Top.html

Correspondence Analysis of Codon Usage
http://www.molbiol.ox.ac.uk/cu/

DNASIS - genetic systems: plasmid map drawing, primer design, multiple sequence alignment etc. .
http://www.oligo.net/dnasis.htm

GCUA: General Codon Usage Analysis. This program is useful for carrying out analysis of variation in codon usage within a collection of genes such as might be found in a complete genome. Some of the most popular multivariate analysis methods are implemen
http://bioinf.nuim.ie/gcua/

Lasergene 99 is the complete sequence analysis system for Windows and Macintosh
http://www.dnastar.com/

MacStripe is the ideal tool for anyone who wants to explore potential alpha-helical coiled coils in the sequence oftheir protein. With a full Macintosh interface, the results of analyses can easily be exported to other software.
http://www.york.ac.uk/depts/biol/units/coils/coilcoil.html

MatInspector - a tool for detection of transcription factor binding sites.
http://www.gsf.de/biodv/matinspector.html

NHGRI WebBLAST is a suite of software tools to aid in viewing and organizing genetic sequencing data.
http://research.nhgri.nih.gov/software/webblast/

Phrap and Phred for Windows, MacOS, Linux, and Unix - Fast sequence assembly and better base calling.
http://www.phrap.com/index.htm

SeqPup - a biosequence editor and analysis program.It includes links to network services and external analysis programs.
http://iubio.bio.indiana.edu/soft/molbio/seqpup/java/seqpup-doc.html

TeXshade is an alignment shading LaTeX macro package for processing multiple sequence alignments. It provides special shading modes featuring functional aspects, e.g. charge, and a plenitude of commands for handling shading colors, text styles and labels.
http://homepages.uni-tuebingen.de/beitz/tse.html