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CLC Gene Workbench is a software suite with a range of advanced and integrated DNA sequence analysis tools - among others, assembly of DNA sequence data, advanced primer design, and molecular cloning. Available for Windows, Mac OS X, and Linux.
http://www.clcbio.com/index.php?id=27

DNASIS - genetic systems: plasmid map drawing, primer design, multiple sequence alignment etc. .
http://www.oligo.net/dnasis.htm

MacPlasmap Pro contains a comprehensive set of functions for advanced desktop cloning, plasmid map drawing, and sequence analysis.
http://www.cgcsci.com/#MacPlasmapPro

MacPlasmap version 2.1 is an easy-to-use color drawing program for preparation of circular and linear plasmid maps.
http://www.cgcsci.com/

MacVector - sequence analysis package for the Macintosh, provides sequence editing, PCR primer analysis, Internet database searches, protein analysis.
http://www.accelrys.com/products/macvector/index.html

Plasmid ARTIST® is the first Macintosh® graphic program designed for molecular biologists that creates and produces publication quality plasmid diagrams.
http://www.plasmidartist.com/

Vector NTI Suite is a comprehensive software package for computer support of molecular biology and genetics
http://www.informaxinc.com/products/vntisuite/index.html