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AutoPrime - Primers for expressed sequences. AutoPrime allows the effortless design of primers for Real-Time PCR. It employs the Primer3 software and sequence information from the Ensembl database to automate the process to the highest degree.
http://www.autoprime.de/

CODEHOP: COnsensus-DEgenerate Hybrid Oligonucleotide Primers.PCR primers designed from protein multiple sequence alignments. The program is intended for cases where the protein sequences are distant from each other and degenerate primers are needed.
http://blocks.fhcrc.org/blocks/codehop.html

GeneFisher: Interactive Primer Design
http://bibiserv.techfak.uni-bielefeld.de/genefisher/

Melting - this program computes, for a nucleic acid duplex, the enthalpy, the entropy and the melting temperature of the helix-coil transitions.
http://www.ebi.ac.uk/~lenov/meltinghome.html

OLIGO 6, Primer Analysis Software for Mac and Windows.
http://www.oligo.net

OLIGO 6, Primer Analysis Software for Mac and Windows.
http://www.medprobe.com

Oligo Calculator
http://www.microbiology.adelaide.edu.au/learn/oligcalc.htm

PCR primer selection program based on xprimer.
http://alces.med.umn.edu/webprimers.html

Primer Designer 4 for Windows 95 -- Software to design, analyze, mutate primers.
http://www.scied.com/ses_pd4.htm

Primer Premier: Comprehensive primer design for Windows and Power Macintosh.
http://www.PremierBiosoft.com/

Primer3
http://www-genome.wi.mit.edu/cgi-bin/primer/primer3_www.cgi

Primer3 - PCR primers from nucleotide sequence
http://bioinformatics.weizmann.ac.il/cgi-bin/primer/primer3.cgi

Primerfinder - A tool to design oligonucleotides suitable for PCR within any sequence you specify. Primers are designed to meet your specific needs based on the information entered on the search page.
http://eatworms.swmed.edu/~tim/primerfinder/

Prodoric - prokaryotic database of gene-regulation and molecular networks.
http://www.prodoric.de

Web Primer: DNA and Purpose Entry
http://genome-www2.stanford.edu/cgi-bin/SGD/web-primer

You can easily design primers for PCR and DNA sequencing using our free Web DoPrimer software ! The sequence information you need can be retrieved on-the-fly from the EMBL sequence database.
http://doprimer.interactiva.de/